096 Deconvolution of adult T-cell leukemia/lymphoma with single-cell RNA-seq using frozen archived skin tissue reveals new subset of cancer-associated fibroblast

Adult T-cell Leukemia/Lymphoma (ATLL) is a rare aggressive malignancy associated with human leukemia virus type 1 (HTLV-1) infection. However, little known about the underlying activated molecular pathways at single cell level. Moreover, intercellular communications between tumor microenvironment (TME) and cells in this are currently unknown. Difficulties harvesting fresh tissue clinical setting has hampered our deeper understanding of malignancy. Herein, we examined ATLL using archived frozen after biopsy by single-cell RNA sequencing (scRNA-seq) receptor (TCR) clonal analysis. Highly showed multiple activating pathways, suggesting dynamic evolution Dissecting diverse types comprising TME led to identification novel subset cancer-associated fibroblast which enriched epidermal growth factor (EGFR)-related transcripts. These findings suggest potential targeted therapeutic pathway better treat neoplasm.